To achieve a reliable conclusion a variety of different controls were used. This was done to make sure that the antibodies and dyes that were used were actually conclusive. Thus, there was no nonspecific cell binding. The cells used were all from the same species of mice, balb/c mice. This was done in order to keep a semi-constant
This figure shows the end result of all the dyes and antibodies. These images were all taken after the antibodies and dyes. All the dye-ing concluded was that the cancer cells do in fact have TFF2 in them and that MDSCs have the CXCR4 receptor protein.